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Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...
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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
Ion-Exchange Chromatography01:09

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Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

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Published on: June 12, 2016

Continuous counter-current extraction on an industrial sample using dual-flow counter-current chromatography.

Remco van den Heuvel1, Ben Mathews, Stephane Dubant

  • 1Brunel Institute for Bioengineering, Brunel University, Uxbridge, UK. remco.van.den.heuvel@brunel.ac.uk

Journal of Chromatography. A
|February 13, 2009
PubMed
Summary

Continuous counter-current chromatography is feasible for industrial liquor separation. Component elution depends on flow rates but not liquor concentration, paving the way for pharmaceutical applications.

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Area of Science:

  • Chemical Engineering
  • Separation Science

Background:

  • Industrial liquor separation often relies on batch processes.
  • Continuous chromatography offers potential efficiency gains but faces implementation challenges.

Purpose of the Study:

  • To evaluate the feasibility of continuous counter-current chromatography for industrial liquor separation.
  • To investigate the impact of flow rates and liquor concentration on component elution.

Main Methods:

  • Batch and continuous separations were conducted using a custom-built continuous counter-current extraction centrifuge.
  • Flow regimes and reaction liquor concentrations were systematically varied.

Main Results:

  • Component elution was found to be dependent on the flow rates of both upper and lower phases.
  • Component elution was not significantly affected by the concentration of the injected reaction liquor.
  • Continuous processing using the counter-current chromatography centrifuge was demonstrated as feasible.

Conclusions:

  • Continuous counter-current chromatography is a viable method for industrial liquor separation.
  • This technology advancement can enhance efficiency and accessibility for the pharmaceutical industry.