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Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Confocal Fluorescence Microscopy

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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: Jun 25, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

A fluorescence scanning electron microscope.

Takaaki Kanemaru¹, Kazuho Hirata, Shin-Ichi Takasu

¹Morphology and Core Unit, Kyushu University Hospital, Kyushu, Japan.

Ultramicroscopy

|February 13, 2009

Summary

This summary is machine-generated.

Researchers developed a novel fluorescence scanning electron microscope (FL-SEM) for combined imaging. This simple, practical tool integrates fluorescence and electron microscopy, simplifying correlative imaging in biological research.

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Area of Science:

Biological imaging
Microscopy techniques
Correlative imaging

Background:

Fluorescence microscopy (FM) visualizes molecular localization.
Electron microscopy (EM) provides ultrastructural details.
Current correlative imaging requires separate instruments.

Purpose of the Study:

To develop a hybrid instrument combining FM and EM.
To simplify correlative fluorescence and electron microscopy.

Main Methods:

A scanning electron microscope (SEM) was integrated with a fluorescence digital camera unit.
The hybrid instrument was named fluorescence SEM (FL-SEM).
Fluolid and Alexa Fluor dyes were used for sample labeling.

Main Results:

Successful demonstration of the FL-SEM's capability.
Achieved correlative fluorescence and electron microscopy images.
The FL-SEM proved to be a simple and practical tool.

Conclusions:

The FL-SEM offers a streamlined approach to correlative imaging.
This hybrid microscopy technique enhances biological research capabilities.
FL-SEM is a valuable tool for visualizing molecular and ultrastructural information simultaneously.