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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

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Highly sensitive amperometric biosensor based on a biocompatible calcium phosphate cement.

M Sánchez-Paniagua López¹, F Tamimi, E López-Cabarcos

¹Sección Departamental de Química Analítica, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain.

Biosensors & Bioelectronics

|February 13, 2009

Summary

This study introduces a novel enzymatic biosensor using brushite cement for detecting phenolic compounds. The brushite-based biosensor demonstrates high sensitivity and rapid response in various solutions, enabling successful real-sample analysis.

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Area of Science:

Biomaterials Science
Electrochemistry
Biosensor Technology

Background:

Brushite, a biocompatible calcium phosphate mineral, exhibits solid electrolyte properties.
Enzymatic amperometric biosensors offer sensitive detection of various analytes.
Phenolic compounds are prevalent environmental pollutants and indicators of water quality.

Purpose of the Study:

To develop a novel enzymatic amperometric biosensor utilizing brushite cement.
To immobilize tyrosinase (PPO) on brushite cement for phenolic compound detection.
To optimize and evaluate the biosensor's performance in aqueous and non-aqueous solutions.

Main Methods:

Immobilization of tyrosinase (PPO) onto a brushite cement layer on a glassy carbon electrode.
Cross-linking of the immobilized enzyme using glutaraldehyde (GA).
Optimization of immobilization variables (GA time, PPO/brushite ratio, film thickness) and operational parameters (pH, temperature, potential).

Main Results:

The biosensor achieved a rapid response time of 12 seconds.
A wide linear range was observed (0.001-3 µM in PBS, 0.007-2 µM in acetonitrile/PBS).
Low detection limits (1-2 nM) and high sensitivity (28.6-46.6 A M⁻¹ cm⁻²) were recorded.

Conclusions:

The developed brushite-cement-based biosensor is effective for detecting phenolic compounds.
The biosensor demonstrates robust analytical properties in both aqueous and non-aqueous media.
Successful application in real sample analysis highlights its practical utility.