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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Chromatin Structure and RNA Splicing

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: Jun 25, 2026

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

Structural mapping of spliceosomes by electron microscopy.

Reinhard Lührmann¹, Holger Stark

¹Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

Current Opinion in Structural Biology

|February 13, 2009

Summary

This summary is machine-generated.

Accurate removal of introns by the spliceosome is crucial for gene expression. Advances in electron microscopy and analysis tools will enable higher resolution structural studies of this dynamic molecular machine.

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Area of Science:

Molecular Biology
Structural Biology
Genetics

Background:

Eukaryotic gene expression involves transcribing pre-mRNAs containing noncoding introns.
The spliceosome, a large and dynamic macromolecular machine, is responsible for the precise removal of introns.
Understanding spliceosome structure is essential for comprehending gene expression regulation.

Purpose of the Study:

To highlight the challenges in structural studies of the spliceosome due to its size and dynamic nature.
To emphasize the role of electron microscopy in visualizing spliceosome architecture.
To discuss recent technological advancements facilitating higher resolution structural analysis.

Main Methods:

Utilizing electron microscopy (EM) to investigate the overall shape and architecture of spliceosomes.
Employing EM to map the locations of spliceosome subunits and RNA components.
Leveraging recent improvements in sample preparation, EM instrumentation, and computational analysis software.

Main Results:

Electron microscopy provides insights into the large-scale organization of spliceosomes.
The technique allows for the localization of individual components within the spliceosome complex.
Recent advancements are poised to significantly enhance the resolution of spliceosome structural data.

Conclusions:

Structural studies of the spliceosome are critical for understanding gene expression.
Electron microscopy is a powerful technique for elucidating spliceosome structure and dynamics.
Future advancements in EM technology and analysis will drive higher resolution structural insights into spliceosome function.