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Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
Published on: May 22, 2012
[The polymerase chain reaction PCR and its use in genetic diagnosis]
1Abteilung Humangenetik, Medizinischen Akademie Magdeburg.
Abstract:
In genomic diagnosis the ensemble of techniques has been recently expanded by the powerful method of the polymerase chain reaction (PCR). Using pairs of synthetic oligonucleotides for priming of synthesis and a thermoresistant DNA polymerase a millionfold amplification of target DNA sequences from patients provides DNA fragments for following investigations such as electrophoresis. The paper presents some examples of PCR application for diagnosis in cystic fibrosis and Duchenne muscular dystrophy.
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