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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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Related Experiment Video

Updated: Jun 25, 2026

Single-cell Profiling of Developing and Mature Retinal Neurons
10:20

Single-cell Profiling of Developing and Mature Retinal Neurons

Published on: April 19, 2012

Detection of gene expression in an individual cell type within a cell mixture using microarray analysis.

Penelope A Bryant1, Gordon K Smyth, Roy Robins-Browne

  • 1Department of Paediatrics, The University of Melbourne, Royal Children's Hospital Melbourne, Parkville, Victoria, Australia.

Plos One
|February 13, 2009
PubMed
Summary
This summary is machine-generated.

Analyzing gene expression in mixed cells like peripheral blood mononuclear cells (PBMC) can obscure subtle changes. However, major gene expression shifts in monocytes are still detectable in PBMC mixtures.

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Cell Type-specific Gene Expression Profiling in the Mouse Liver

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Related Experiment Videos

Last Updated: Jun 25, 2026

Single-cell Profiling of Developing and Mature Retinal Neurons
10:20

Single-cell Profiling of Developing and Mature Retinal Neurons

Published on: April 19, 2012

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

Area of Science:

  • Immunology
  • Molecular Biology
  • Genomics

Background:

  • Designing microarray experiments requires choosing between single-cell type or mixed-cell analysis for gene expression.
  • Lipopolysaccharide (LPS) stimulation is a common method to induce gene expression changes in monocytes.

Purpose of the Study:

  • To quantify how many LPS-induced monocyte genes are detectable in peripheral blood mononuclear cells (PBMC).
  • To determine the extent to which non-monocyte cells dilute or mask gene expression changes in monocytes within a PBMC mixture.

Main Methods:

  • Human PBMC were stimulated with LPS.
  • Monocytes were isolated using positive (Mono+) or negative (Mono-) selection.
  • The non-monocyte fraction (MonoD) was analyzed.
  • RNA from stimulated and unstimulated samples was hybridized to oligonucleotide microarrays.

Main Results:

  • Gene expression in PBMC showed a positive correlation with both Mono+ (0.77) and Mono- (0.61-0.67) samples.
  • About half of the statistically significant LPS-induced monocyte gene expression changes were not detected in PBMC due to dilution effects.
  • However, 97% of genes with a four-fold or greater change and nearly all top 100 differentially expressed monocyte genes were detected in PBMC.

Conclusions:

  • Non-responding cells in mixtures dilute or obscure subtle gene expression changes in specific cell types.
  • Analyzing individual cell types may be unnecessary for studies focusing only on highly differentially expressed genes.