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Related Experiment Video

Updated: Jun 25, 2026

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

Evaluation of cariogenic bacteria.

Fusao Nishikawara¹, Yoshiaki Nomura, Susumu Imai

¹Department of Preventive Dentistry and Public Health, School of Dental Medicine, Tsurumi University,Japan.

European Journal of Dentistry

|February 13, 2009

Summary

A modified Mitis-Salivalius Bacitracin (MSB) agar medium, termed mMSB-kit, demonstrates superior sensitivity for detecting Mutans streptococci (MS) compared to standard methods and commercial kits. This enhanced detection aids in evaluating caries risk more effectively.

Keywords:

Commercial kits Culture methods Streptococci mutans Streptococcus sobrinus

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Published on: December 14, 2016

Area of Science:

Microbiology
Dental Caries Research
Diagnostic Microbiology

Background:

Mutans streptococci (MS) are key indicators of caries risk.
Commercial kits like Dentocult(TM) and CRT(TM) offer convenient MS evaluation.
Existing methods, including instruction manuals, have limitations in ease of use and accuracy.

Purpose of the Study:

To evaluate the utility of a modified Mitis-Salivalius Bacitracin (MSB) agar medium.
To compare the modified MSB agar medium with standard MSB agar and commercial kits for MS detection.
To establish a convenient kit (mMSB-kit) based on the modified MSB agar medium.

Main Methods:

Detection of MS in stimulated saliva from 27 subjects using MSB agar, modified MSB agar, and commercial kits.
Evaluation of laboratory and clinically isolated MS strains.
Comparison of MS ratios using ELISA.

Main Results:

The mMSB-kit, utilizing modified MSB agar, showed significantly higher detection rates for MS compared to standard MSB agar medium (P<.01) and commercial kits, Dentocult SM(TM) (P<.05).
Some samples abundant in MS were not detected by a commercial kit.
Modified MSB agar medium demonstrated increased sensitivity for MS detection.

Conclusions:

Modified MSB agar medium offers superior sensitivity for MS detection over standard MSB agar.
The mMSB-kit provides a simple and effective tool for evaluating MS.
The mMSB-kit can significantly contribute to assessing caries risk.