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Related Experiment Video

Updated: Jun 25, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Antibody-based microarrays.

Christer Wingren¹, Carl A K Borrebaeck

¹Department of Immunotechnology, Lund University, Lund, Sweden.

Methods in Molecular Biology (Clifton, N.J.)

|February 13, 2009

Summary

Antibody-based microarrays offer powerful proteomic analysis for detailed protein expression profiling. This technology enables multiplexed, ultra-sensitive assays for biomarker discovery and disease diagnostics.

Area of Science:

Proteomics
Biotechnology
Analytical Chemistry

Background:

Antibody-based microarrays are an emerging proteomic technology.
They enable rapid and detailed expression profiling of protein analytes in complex samples.
Miniaturized arrays can be printed with numerous specific antibodies.

Purpose of the Study:

To review antibody-based microarray technology.
To discuss its applications in various fields.
To highlight its potential in proteomics research.

Main Methods:

Printing miniaturized micro- and nanoarrays with specific antibodies.
Performing multiplexed and ultra-sensitive assays.
Generating array images for protein expression profiling.

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Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Related Experiment Videos

Last Updated: Jun 25, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Published on: July 26, 2019

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Main Results:

Detailed expression profiles and high-resolution proteome portraits can be generated.
Minute sample amounts are consumed due to multiplexed assays.
Array images are converted into protein expression maps.

Conclusions:

Antibody-based microarrays provide unique opportunities for disease proteomics and biomarker discovery.
This technology supports disease diagnostics and patient stratification.
It is a promising tool for advancing proteomic research.