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Updated: Jun 25, 2026

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
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Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Published on: February 25, 2021

Structural analysis of glycosaminoglycans and proteoglycans by means of Raman microspectrometry

Richard Ellis1, Ellen Green, C Peter Winlove

  • 1School of Physics, University of Exeter, Exeter, United Kingdom. R.E.Ellis@exeter.ac.uk

Connective Tissue Research
|February 13, 2009
PubMed

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Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
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