Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proportionate universalism intervention is effective for tackling nutritional social gradient in adolescents: the PRALIMAP-INÈS mixed randomised trial.

Public health·2023
Same author

Economic evaluation of a school-based strategy to prevent overweight and obesity in French adolescents: insights from the PRALIMAP randomised trial.

Public health·2023
Same author

Individual inorganic nanoparticles: preparation, functionalization and in vitro biomedical diagnostic applications.

Journal of materials chemistry. B·2020
Same author

The effect of vehicle on skin absorption of Mg<sup>2+</sup> and Ca<sup>2+</sup> from thermal spring water.

International journal of cosmetic science·2020
Same author

Formulation of survival acceptor medium able to maintain the viability of skin explants over in vitro dermal experiments.

International journal of cosmetic science·2019
Same author

Obesity phenotype and patient-reported outcomes in moderate and severe chronic kidney disease: a cross-sectional study from the CKD-REIN cohort study.

Quality of life research : an international journal of quality of life aspects of treatment, care and rehabilitation·2019

Related Experiment Video

Updated: Jun 25, 2026

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
21:16

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

Published on: July 13, 2009

rhEGF microsphere formulation and in vitro skin evaluation.

L Al Haushey1, M A Bolzinger, H Fessi

  • 1CNRS, UMR 5007, LAGEP, Université Lyon, Villeurbanne, France.

Journal of Microencapsulation
|February 13, 2009
PubMed
Summary

This study developed epidermal growth factor (rhEGF)-poly-epsilon-caprolactone microspheres for sustained skin delivery. The microspheres effectively encapsulated rhEGF, showing potential for localized, long-term release in skin layers.

More Related Videos

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging
11:07

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

Published on: November 24, 2021

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
08:49

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Related Experiment Videos

Last Updated: Jun 25, 2026

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
21:16

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

Published on: July 13, 2009

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging
11:07

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

Published on: November 24, 2021

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
08:49

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Area of Science:

  • Biomaterials Science
  • Dermatology
  • Drug Delivery Systems

Background:

  • Epidermal growth factor (rhEGF) promotes skin healing and regeneration.
  • Effective delivery of rhEGF to skin layers is crucial for therapeutic outcomes.
  • Poly-epsilon-caprolactone (PCL) is a biodegradable polymer suitable for microparticle formulation.

Purpose of the Study:

  • To optimize the encapsulation process for rhEGF within PCL microspheres.
  • To characterize the physical properties and rhEGF encapsulation efficiency of the microspheres.
  • To evaluate the in vitro release kinetics and skin absorption of rhEGF from the PCL microspheres.

Main Methods:

  • Optimized protein encapsulation process.
  • Formulation of rhEGF-poly-epsilon-caprolactone (PCL) microspheres.
  • Characterization of microsphere size and encapsulation efficiency.
  • In vitro release studies using McIlvaine buffered solution.
  • Ex vivo skin absorption studies using the Franz cell method on human skin.

Main Results:

  • Microspheres achieved a mean size of 3.8 microm +/- 0.2.
  • Encapsulation efficiency was 41.9% +/- 2.6, with approximately 70% rhEGF recovery.
  • Sustained in vitro release of rhEGF over 4 days was observed.
  • In skin absorption studies, 20% rhEGF was released after 24 h, with microsphere accumulation in the stratum corneum.

Conclusions:

  • rhEGF-PCL microparticles are promising for sustained, localized delivery of rhEGF to skin.
  • The microspheres act as a reservoir, potentially enhancing therapeutic effects in skin layers.
  • The optimized formulation demonstrates potential for advanced dermatological applications.