Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new method for measuring auricular inflammation in the mouse.

A Colorado1, J T Slama, W B Stavinoha

  • 1University of Texas Health Science Center, San Antonio Department of Pharmacology 78284-7764.

Journal of Pharmacological Methods
|August 1, 1991
PubMed
Summary

The croton oil ear test is a common method for evaluating anti-inflammatory drugs. This study reviews its limitations and discusses alternative measurement techniques for more reliable drug efficacy assessment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diabetes, undernutrition, migration and indigenous communities: tuberculosis in Chiapas, Mexico.

Epidemiology and infection·2019
Same author

An empirical approach to estimation of critical energies by using a quadrupole ion trap.

Journal of the American Society for Mass Spectrometry·2013
Same author

Mouse brain concentrations of 3-methoxytyramine and normetanephrine: a comparison of methods of sacrifice.

Neurochemistry international·2010
Same author

Immediate effects of catecholamines on plasma fibrinogen in conscious and anaesthetized dogs.

British journal of pharmacology and chemotherapy·2008
Same author

PTEN and myotubularin phosphoinositide phosphatases: bringing bioinformatics to the lab bench.

Current opinion in cell biology·2001
Same author

Role of NAD(+) in the deacetylase activity of the SIR2-like proteins.

Biochemical and biophysical research communications·2000

Area of Science:

  • Pharmacology
  • Drug Discovery
  • Inflammation Research

Background:

  • The croton oil ear test is a standard in vivo model for assessing topical anti-inflammatory drug efficacy.
  • Inflammation is induced by topical croton oil application in rodents, with drug effectiveness gauged by reduced swelling.
  • Traditional methods involve animal sacrifice and ear weighing, or caliper/micrometer measurements with potential for error.

Purpose of the Study:

  • To review the established croton oil ear test for anti-inflammatory drug screening.
  • To highlight the limitations of current measurement techniques, including animal sacrifice and potential operator bias.
  • To discuss the need for improved methodologies in evaluating anti-inflammatory drug activity.

Main Methods:

  • Review of existing literature on the croton oil ear test and its variations.

Related Experiment Videos

  • Analysis of measurement techniques including ear weighing, caliper, and micrometer measurements.
  • Discussion of the drawbacks associated with each method, such as animal use and measurement accuracy.

    • Main Results:

    • The croton oil ear test is widely adopted but requires significant animal use and chemical quantities for time-course studies.
    • Ear thickness measurements using calipers or micrometers allow for repeated measures but lack standardized pressure application.
    • Concerns exist regarding operator error, bias, and fluid displacement when using calipers for ear thickness assessment.

    Conclusions:

    • The croton oil ear test, while common, presents challenges in terms of animal welfare and measurement precision.
    • Alternative or refined methods are needed to improve the reliability and efficiency of anti-inflammatory drug evaluation.
    • Further research into non-invasive and standardized techniques is crucial for accurate drug screening.