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Related Concept Videos

Transgenic Organisms00:53

Transgenic Organisms

Overview
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: Jun 25, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Developing tTA transgenic rats for inducible and reversible gene expression.

Hongxia Zhou1, Cao Huang, Min Yang

  • 1Department of Pathology, Anatomy & Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA.

International Journal of Biological Sciences
|February 14, 2009
PubMed
Summary

We developed new transgenic rats for inducible gene expression using tetracycline-controlled transactivator (tTA). Doxycycline (Dox) effectively controls gene expression, allowing for precise ON/OFF switching and suppression during development.

Keywords:
DoxycyclineLRRK2Leucine Rich Repeat Kinase 2RatstTAtetracycline-controlled transactivatortransgenic

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Last Updated: Jun 25, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

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Published on: May 17, 2020

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
09:35

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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Area of Science:

  • Genetics and Molecular Biology
  • Animal Models
  • Gene Regulation

Background:

  • Conditional gene expression is crucial for studying gene function.
  • Existing methods may lack precise temporal or spatial control.
  • Transgenic rat models are valuable for disease research.

Purpose of the Study:

  • To create transgenic rat lines for conditional gene expression.
  • To investigate Doxycycline (Dox) regulation of gene expression dynamics.
  • To establish a tool for developing genetic rat models of human diseases.

Main Methods:

  • Generation of transgenic rats carrying the tetracycline-controlled transactivator (tTA) gene.
  • Utilizing a ubiquitous promoter for widespread tTA expression.
  • Administering Doxycycline (Dox) at varying doses and schedules to regulate gene expression.

Main Results:

  • Widespread tTA expression was achieved without toxicity.
  • Dox dose-dependently suppressed tTA-activated reporter gene expression.
  • A Dox administration regimen enabled rapid ON/OFF gene expression switching.
  • Dox administration to pregnant rats suppressed offspring gene expression.

Conclusions:

  • The tTA transgenic rat enables inducible and reversible gene expression.
  • This system offers precise control over gene activity.
  • It is a valuable tool for creating genetic rat models of human diseases.