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Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
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Published on: February 4, 2016

Voltage-gated metal-enhanced fluorescence.

Yongxia Zhang1, Kadir Aslan, Chris D Geddes

  • 1The Institute of Fluorescence, University of Maryland Biotechnology Institute, 701 East Pratt St, Baltimore, MD 21202, USA.

Journal of Fluorescence
|February 14, 2009
PubMed
Summary
This summary is machine-generated.

Electrical current impacts surface plasmon amplification of fluorescence. In Silver Island Films, applied direct current modulates fluorescence enhancement, with effects varying based on film conductivity and surface plasmon availability for fluorophore coupling.

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Area of Science:

  • Plasmonics
  • Surface Science
  • Fluorescence Spectroscopy

Background:

  • Surface plasmons play a crucial role in enhancing optical phenomena.
  • Metal-enhanced fluorescence (MEF) is a key application of surface plasmon resonance.
  • The influence of electrical fields on plasmonic properties is an area of active research.

Purpose of the Study:

  • To investigate the effect of applied direct current on surface plasmon-mediated fluorescence enhancement.
  • To understand how electrical current influences the interaction between surface plasmons and fluorophores.
  • To explore the relationship between film conductivity and metal-enhanced fluorescence (MEF).

Main Methods:

  • Fabrication of Silver Island Films (SIFs) with varying electrical resistance.
  • Application of direct current across the SIFs.
  • Measurement of fluorescence enhancement as a function of applied current and film properties.

Main Results:

  • Applied direct current perturbs fluorescence enhancement in SIFs.
  • In just-continuous films, fluorescence enhancement is gated by applied current due to limited surface plasmon availability for dipole-coupling.
  • In thicker, low-resistance films, MEF is less perturbed due to abundant charge carriers facilitating surface plasmon formation.

Conclusions:

  • Electrical current provides a tunable mechanism to control surface plasmon amplification of fluorescence.
  • The degree of perturbation depends on the film's electrical resistance and the availability of surface plasmons.
  • This work offers insights into electrical control of plasmon-enhanced optical signals.