Non-phasic discharge and osmoresponsiveness of cat magnocellular neuroendocrine cells

Abstract Repetitive bursting (phasic firing) generated endogenously by magnocellular neuroendocrine cells (MNCs) in the rat facilitates systemic release of vasopressin from axon terminals in the neurophypophysis. However, little is known of how MNCs function in other mammals. Using coronal slices of hypothalamus we studied the firing behaviour and intrinsic membrane properties of homologous neurons in the cat supraoptic nucleus where vasopressinergic MNCs outnumber oxytocinergic cells. Less than 1% of units recorded in cat supraoptic nuclei (2 of 270) spontaneously fired in a phasic mode compared to 39% in the rat (90 of 230). A discrete level of steady current across the extracellular recording micropipette promoted phasic firing in 66 of 152 non-phasic units tested in rat supraoptic nuclei, but no phasic activity in 189 units from the cat. One or several stimuli applied dorsal to supraoptic nuclei triggered a single burst (afterdischarge) in 115 of 180 MNC units from the rat, whereas none of 173 MNC units tested in the cat fired an afterdischarge. Intracellular recordings from 56 feline MNCs revealed that unlike the rat, spike depolarizing afterpotentials were absent in all cells. This explains both the absence of phasic firing and the inability to trigger regenerative bursts in the intact cat. The possible Osmoresponsiveness of cat MNCs was examined using unit recording. These units reversibly increased their firing rate as osmolality was elevated with mannitol or NaCl (10 to 100 mOsm/kg), comparable to rat units. However, in no case did hyperosmotic conditions elicit phasic firing. We conclude that cat MNCs lack a regenerative burst capability but that unit Osmoresponsiveness is comparable to rat MNC units. We hypothesize that since the kidney of the cat normally functions at high efficiency in terms of water resorption, there may be little need for the rapid and pronounced elevation in vasopressin release evoked by phasic firing.