Neonatal exposure to gonadal hormones affects the development of monoamine systems in rat cortex

Abstract The effects of neonatal gonadectomy of male, and testosterone propionate treatment of female rat pups on levels of monoamines and metabolites in the cerebral cortex were assessed using high-performance liquid chromatography with electrochemical detection. In Experiment 1, pups were killed at 0, 4, 10 and 21 days of age and the anterior and posterior portions of cortex in each hemisphere were removed. At 21 days of age the levels of dopamine in anterior cortex were higher in males and testosterone propionate-treated females than in females and gonadectomized males. However, dopaminergic activity developed earlier in females than in males and the gonadal hormone manipulations shifted the pattern of development to that of the other sex. In Experiment 2, the effects of these same gonadal hormone manipulations on the uptake, metabolism and storage capacity of catecholamine neurons in the cingulate, agranular insular, parietal and occipital cortex were estimated at 4 and 10 days of age by considering the difference between measured catecholamines in animals pretreated with vehicle or 2.5 mg/kg reserpine and then given 100 mg/kg L-DOPA. Again, the data indicated earlier development of catecholamine neurons in females, especially in the agranular insular cortex. Dopamine was found to account for group differences; for when dopamine levels alone were considered it was found that, at 4 days of age, females had the highest levels in every area with the exception of the occipital cortex where gonadectomized males had equally high levels. These data suggest a mechanism that might account for sex differences in the development of specific cortical regions.