Chapter 10. Estimating nuclear mRNA decay in Saccharomyces cerevisiae

Manfred Schmid1, Mathieu Rougemaille, Dominico Libri

  • 1Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, University of Aarhus, Aarhus C, Denmark.

Methods in Enzymology
|February 14, 2009
PubMed
Summary

Nuclear RNA decay is crucial in eukaryotes, removing aberrant transcripts and regulating gene expression. This study details methods to measure nuclear mRNA degradation in yeast, focusing on the HSP104 transcript.

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