Related Experiment Video
Updated: Jun 25, 2026

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
Published on: December 13, 2014
Chapter 10. Estimating nuclear mRNA decay in Saccharomyces cerevisiae
Manfred Schmid1, Mathieu Rougemaille, Dominico Libri
1Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, University of Aarhus, Aarhus C, Denmark.
Nuclear RNA decay is crucial in eukaryotes, removing aberrant transcripts and regulating gene expression. This study details methods to measure nuclear mRNA degradation in yeast, focusing on the HSP104 transcript.
Area of Science:
- Molecular Biology
- Yeast Genetics
- RNA Biology
Background:
- RNA decay occurs significantly in the nucleus of eukaryotic cells.
- Nuclear RNA decay targets non-genic RNAs and aberrant transcripts from regular genes.
- Specific genes utilize nuclear RNA turnover for expression regulation.
Purpose of the Study:
- To present concepts and techniques for estimating nuclear mRNA degradation in Saccharomyces cerevisiae.
- To focus on the nuclear RNA decay pathway of the HSP104 transcript under specific genetic conditions.
Main Methods:
- Utilizing yeast (Saccharomyces cerevisiae) as a model organism.
- Analyzing the nuclear RNA decay of the heat-inducible HSP104 transcript.
- Investigating strains with an inactive THO/Sub2p mRNA export complex.
Main Results:
- Established methodologies for quantifying nuclear mRNA degradation.
- Demonstrated the applicability of these methods to specific transcripts and genetic backgrounds.
Conclusions:
- The presented techniques are adaptable for analyzing various nuclear mRNA degradation scenarios in yeast.
- Provides a framework for studying RNA turnover in the nucleus.
More Related Videos
09:21Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
Published on: October 22, 2018
07:03Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
Published on: January 2, 2018
Related Concept Videos
Nonsense-mediated mRNA Decay
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nonsense-mediated mRNA Decay
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nuclear Export of mRNA
mRNA Stability and Gene Expression
Cis-acting Elements involved in mRNA stability