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[Study on bulblet induction of Fritillaria anhuiensis in vitro]
Jian-ping Xue1, Ai-min Zhang, Mao-lin Geng
1Anhui Key Laboratory of Plant Resources and Biology, Department of Biology, Huaibei Coal Industry Teachers' College, Anhui Province, Huaibei 235000, China. xuejp2000@yahoo.com.cn
Objective:
To study the optimal condition for bulb induction of Fritillaria anhuiensis in vitro.
Method:
Effects of sucrose, salicylic acid, active carbon and 5 degrees C pre-incubation on bulb formation in vitro were investigated by adopting the number of bulb and fresh weight as indexes.
Result:
The number of bulb and fresh weight in medium added with 50 g x L(-1) sucrose were significantly higher than those with other treatments. The medium with different concentrations of salicylic acid showed no significant promotion on callus differentiation. However, 5 g x L(-1) active carbon (AC) treatment was better than other treatments. Meanwhile, a large number of plantlets formed after 5 degrees C pre-incubation for 30-40 days was suitable for bulb formation and growth.
Conclusion:
Bulblet formation was optimal in MS + KT 2 mg x L(-1) + NAA 2 mg x L(-1) + 50 g x L(-1) sucrose +5 g x L(-1) AC after callus pre-incucation at 5 degrees 1 for 30-40 days.
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