Related Experiment Video
Updated: Apr 27, 2026

The Trier Social Stress Test Protocol for Inducing Psychological Stress
Published on: October 19, 2011
Endothelial pulse amplitude testing: feasibility and reproducibility in adolescents
Elif Seda Selamet Tierney1, Jane W Newburger, Kimberlee Gauvreau
1Department of Cardiology, Children's Hospital, Boston, MA 02155, USA. Seda.Tierney@cardio.chboston.org
Objectives:
To test prospectively the reproducibility and feasibility of endothelial pulse amplitude testing (Endo-PAT), a novel Food and Drug Administration-approved technology, in healthy adolescents.
Study Design:
We performed Endo-PAT testing on 2 different days separated by no more than 7 days in 30 healthy fasting adolescents, ages 13 to 19 years, to assess reproducibility and feasibility. The reported level of discomfort, as measured on a pain scale of 1 to 5, was documented.
Results:
The mean difference in paired Endo-PAT indices was 0.12 (95% CI, -0.09-0.33; P = .24; intraclass correlation coefficient, 0.78), and the within-subject variation of Endo-PAT index was 0.16. The Endo-PAT index on test days 1 and 2 were 1.91 +/- 0.57 and 1.78 +/- 0.51 (mean plus or minus SD), respectively. All attempted studies (100%) were completed (95% CI, 88%-100%), and all completed studies (100%) could be analyzed (95% CI, 88%-100%). The median pain score was 1 on both days.
Conclusion:
In healthy adolescents, Endo-PAT is feasible and has excellent reproducibility. This technology may provide an easy and reliable means of assessing endothelial function in the pediatric population.
Related Concept Videos
Self-Evaluation: Self-Enhancement and Self-Verification
General State of Stress
Specifically, consider a tetrahedral element where one face, labeled XYZ, is perpendicular to the line OA, and the remaining faces align with the coordinate axes with point O as the origin. At any point, such as point O, the stress tensor can be used to determine the stress...
Ethnic Identity within a Larger Culture
Introduction to Stress and Lifestyle
Psychological Responses to Stress
Stress and Mental Health
Individuals with depression often experience challenges in both their personal and professional...

