Related Experiment Video
Updated: Jun 25, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
Published on: December 9, 2022
Computational modeling analyses of RNA secondary structures and phylogenetic inference of evolutionary conserved 5S
Vijai Singh1, Pallavi Somvanshi
1Bioinformatics Centre, Biotech Park, Sector-G Jankipuram, Lucknow 226021, Uttar Pradesh, India.
Abstract:
Bacteria are unicellular, ubiquitous microorganisms which grow on soil, acidic hot springs, radioactive wastes, etc. The genome of bacteria constitutes species specific conserved region. The 5S rRNA is one of the most conserved region determined in each bacteria and the size ranges between 110 and 148 bp. On this basis phylogenetic study of 37 bacterial strains was done which results in formation of seven clades and furthermore RNA secondary structure from each clade was made. The lowest free energy (delta G) of the 5S rRNA may divulge the most primitive bacteria and slow changes occurs throughout the evolution whereas the higher free energy indicates less stability during the evolution. The RNA secondary structure may provide new insights to understand bacteria evolution and stability.
Related Concept Videos
RNA Structure
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Three-Domain System of Life
Ribosomal RNA Synthesis
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

