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Preliminary evaluation of sensititre system for identifying gram-negative bacilli
G Mucignat1, G L Tarabini-Castellani, L Pascoli
1Servizio di Microbiologia ed Immunologia, Ospedale Civile di Pordenone.
Microbiologica
|July 1, 1991
Summary
The Sensititre System accurately identifies Gram-negative bacilli, with 18-hour incubation yielding 82.5% concordance. This automated method offers 100% reproducibility for clinical microbiology identification.
Area of Science:
- Clinical Microbiology
- Bacterial Identification
- Diagnostic Systems
Background:
- Accurate identification of Gram-negative bacilli is crucial for effective patient treatment.
- The Sensititre System offers an automated approach to bacterial identification.
Purpose of the Study:
- To evaluate the performance of the automated Sensititre System for identifying Gram-negative bacilli.
- To compare Sensititre System results with the established API 20E method.
Main Methods:
- The Sensititre System was used to identify 120 clinical isolates and 12 ATCC strains.
- Identifications were performed after 5-hour and 18-hour incubations.
- Results were compared against the API 20E reference method.
Main Results:
- 18-hour incubation achieved 82.5% concordance with API 20E, compared to 65% at 5 hours.
- 100% reproducibility was observed for both incubation times.
- Identification accuracy for ATCC strains was 66.6% at 5 hours and 100% at 18 hours.
Conclusions:
- The Sensititre System demonstrates high accuracy and reproducibility for Gram-negative bacilli identification.
- An 18-hour incubation period significantly improves the concordance rate.
- The Sensititre System is a reliable tool for clinical microbiology laboratories.