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Identification of mutation sites on Delta12 desaturase genes from Mortierella alpina 1S-4 mutants
Eiji Sakuradani1, Takahiro Abe, Kenji Matsumura
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Journal of Bioscience and Bioengineering
|February 17, 2009
Abstract:
The mutation sites on the Delta12 desaturase gene in Mortierella alpina Delta12 desaturase-defective mutants SR88, TM912, and Mut48 accumulating Mead acid were identified. Each mutation resulted in an amino acid replacement (H116Y and P166L) in the Delta12 desaturase gene from SR88 and Mut48, respectively.
