[Turbidostat culture of yeast during transition from a resting state to active growth]

The age components of a Saccharomyces cerevisiae 14 culture and the kinetics of its growth were studied afer the quiescent state at the onset active growth. The following factors induced the quiescent state: the cessation of a chemostat flow for 24 h, growth inhibition with 2,4-dinitrophenol (DNP) for 24 h, the storage of a culture growing on agar in a refrigerator during 24 h. The process of transition from the point of growth activation to the maximum rate of growth was then studied in turbidostat. This process took the shortest time in a refrigerated culture as well as in a culture that had been limited with a phosphorus source and in a culture limited with a nitrogen source and grown in chemostat at D = 0.26 h-1. The process was longer in cultures that had been either limited with glucose or inhibited with DNP and longest in a chemostat culture limited with a nitrogen source at D = 0.15 and 0.05 h-1. The rate of initial mitosis phases in the yeast is presumed to exert the greatest effect on the duration of this transition process.