Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecularly imprinted plasmonic nanosensors: A reagent-free platform for ultrasensitive, real-time drug detection in complex biofluids.

Talanta·2026
Same author

Molecularly Imprinted Polymer Nanoparticles for Lung-Cancer-Cell-Surface Proteomics.

Polymers·2026
Same author

Development of one-step magnetic assay for the detection of fentanyl.

The Analyst·2025
Same author

Molecularly imprinted polymer-integrated nanozymes for biosensing: advances and prospects.

Journal of materials chemistry. B·2025
Same author

Synthesis of selective fluorescent nanoMIPs via solid-phase imprinting for a homogeneous FRET assay: tryptamine detection in fruits.

Food chemistry·2025
Same author

A dummy template molecularly imprinted polymer-coated fiber array extraction for simultaneous HPLC analysis of eight biogenic amines in fermented horse milk.

Analytica chimica acta·2025

Related Experiment Video

Updated: Jun 25, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Selection of imprinted nanoparticles by affinity chromatography.

António R Guerreiro1, Iva Chianella, Elena Piletska

  • 1Cranfield Health, Cranfield University, College Road, Cranfield, Bedfordshire, MK43 0AL, UK. a.guerreiro@cranfield.ac.uk

Biosensors & Bioelectronics
|February 17, 2009
PubMed
Summary

Researchers developed high-affinity molecularly imprinted nanoparticles using iniferter polymerization. These nanoparticles mimic natural antibodies, showing excellent binding and discrimination capabilities for target molecules.

More Related Videos

Synthesis of Immunotargeted Magneto-plasmonic Nanoclusters
09:43

Synthesis of Immunotargeted Magneto-plasmonic Nanoclusters

Published on: August 22, 2014

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

Related Experiment Videos

Last Updated: Jun 25, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Synthesis of Immunotargeted Magneto-plasmonic Nanoclusters
09:43

Synthesis of Immunotargeted Magneto-plasmonic Nanoclusters

Published on: August 22, 2014

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

Area of Science:

  • Polymer Chemistry
  • Nanotechnology
  • Biomaterials Science

Background:

  • Molecularly imprinted nanoparticles (MINPs) offer potential as synthetic receptors.
  • Developing MINPs with high affinity and selectivity remains a challenge.
  • Existing methods often yield heterogeneous mixtures requiring further purification.

Purpose of the Study:

  • To synthesize soluble MINPs with enhanced affinity and selectivity.
  • To develop a purification strategy for isolating high-affinity MINP fractions.
  • To evaluate the binding characteristics and discrimination ability of purified MINPs.

Main Methods:

  • Synthesis of soluble MINPs using iniferter-initiated polymerization.
  • Size-based separation of nanoparticles via gel permeation chromatography.
  • Affinity-based fractionation to isolate high-affinity MINP populations.

Main Results:

  • Successful synthesis of soluble MINPs.
  • Demonstration of effective size and affinity-based fractionation.
  • Isolated MINP fractions exhibited binding affinities comparable to natural antibodies (Kd ≈ 6.6x10⁻⁸ M).
  • Purified MINPs showed the ability to discriminate between the template and its analogues.

Conclusions:

  • Iniferter-initiated polymerization is a viable method for creating soluble MINPs.
  • Affinity chromatography is effective for purifying MINPs based on binding strength.
  • The developed MINPs show promise as artificial antibodies for molecular recognition applications.