Related Experiment Video
Updated: Jun 25, 2026

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
Published on: November 1, 2017
Increase of hematopoietic progenitor and suppression of endothelial gene expression by Runx1 expression during in
Eiko Sakai1, Kenji Kitajima, Ayuko Sato
1Department of Pathology, School of Medicine and Frontier Biosciences, Osaka University, Yamadaok, Osaka, Japan. esakai@patho.med.osaka-u.ac.jp
Objective:
Runx1 is essential for both the establishment of hematopoiesis during development and maintenance of adult hematopoiesis. To reveal the roles of Runx1, we examined how and when Runx1 functions during development of hematopoiesis, and revealed the genes controlled by Runx1.
Materials And Methods:
A combined in vitro approach involving in vitro hematopoietic differentiation of embryonic stem cells and conditional gene expression of Runx1 was utilized for this study. Then we analyzed the effects of Runx1 on the differentiation and proliferation of hematopoietic cells and carried out DNA microarray analysis.
Results:
Pulse expression of Runx1 prior to the emergence of hematopoietic cells caused immature hematopoietic cell increase but did not have any effects on the induction of hemogenic cells. During this process, the mRNA level of several endothelial cell-specific genes was downregulated.
Conclusion:
Runx1 expression play important roles on the proliferation of emerging immature hematopoietic progenitors or the transition process from endothelial to hematopoietic cells presumably by suppressing the genes related to endothelial phenotype.
More Related Videos
Related Concept Videos
Regulation of Hematopoietic Stem Cells
Multipotency of Hematopoietic Stem Cells
Hematopoiesis
Role of Hematopoietic Growth Factors
Thrombopoietin (TPO), mainly released by the liver,...
Overview of Hematopoiesis
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
Production of Formed Elements
Most HSCs commit to...

