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Updated: Jun 25, 2026

Kupffer Cell Isolation for Nanoparticle Toxicity Testing
Published on: August 18, 2015
[Effect of AT-1 alpha receptor gene silencing on nuclear factor-kappaB activity in hepatic Kupffer cells]
Xu Li1, Ying Meng, Gao-su Zhou
1Department of Emergency Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. mylx99@163.com
Objective:
To investigate the effect of angiotensin II type-1 (AT-1) alpha receptor gene silencing on nuclear factor-kappaB (NF-kappaB) activity in hepatic Kupffer cells.
Methods:
The expression of AT-1 alpha receptors in primary isolated cultured hepatic Kupffer cells was detected by immunohistochemistry. pSilencer/AT-1 alpha receptor siRNA plasmids were transfected into Kupffer cells, which were subsequently exposed to 10(-6) mol/L angiotensin II (Ang II) for 60 min. The changes in the DNA binding activity of NF-kappaB in the cells was assessed using electrophoretic gel mobility shift assay (EMSA).
Results:
AT-1 alpha receptor expression was detected in Kupffer cells. NF-kappaB DNA binding activity was markedly increased in Kupffer cells after Ang II stimulation, and obviously inhibited by transfectiom with pSilencer/AT-1 alpha receptor siRNA plasmid.
Conclusion:
Ang II stimulation of Kupffer cell results in increased activation of NF-kappaB via AT-1 alpha receptor.
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