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Updated: Jun 25, 2026
Isolation of Alveolar Type II Epithelial Cells from Neonatal, Juvenile, and Adult Murine Lungs Adaptable to Infectious Experimental Settings
Published on: December 19, 2025
[Epithelial sodium channel subunit mRNA expression in acutely isolated rat alveolar type II cells]
Wang-mei Zhou1, Lin He, Tao-ping Li
1Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. zhouwangmei01@163.com
Objective:
To investigate the epithelial sodium channel (ENaC) subunit mRNA expression in acutely isolated rat alveolar type II (ATII) cells.
Methods:
Acutely isolated ATII cells from 20 SD rats were purified and ENaC alpha, beta, gamma-subunit mRNA levels were determined by reverse transcription-polymerase chain reaction (RT-PCR).
Results:
mRNA expressions of all the subunits were detected in the ATII cells, and ENaC alpha-subunit mRNA showed significantly higher expression than beta- and gamma-subunit mRNAs, and the expressions of the latter two mRNAs were comparable.
Conclusion:
As the predominant ENaC subunit expressed at the mRNA level in rat ATII cells, the alpha-subunit of ENAC plays an important role in alveolar fluid clearance.
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