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Published on: June 16, 2023
[MTT assay for detecting 5-fluorouracil chemosensitivity of human breast carcinoma cell line]
Fang-xuan Han1, Hui Lin, Ling Ru
1Department of Pharmacy, People's Hospital of Hainan Province, Haikou 570311, China. hfx1223@163.com
Objective:
To assess the feasibility of MTT colorimetric assay for testing the in vitro chemosensitivity of breast cancer cells to 5 fluorouracil (5-Fu).
Methods:
The chemosensitivity of human breast carcinoma cell lines MCF-7 and MDA-MB-435S to 5-Fu at different concentrations was evaluated with MTT assay.
Results:
5-FU treatment resulted in dose-dependent growth inhibition of the breast cancer cells with both low and high metastatic capacities.
Conclusions:
MTT assay may help select appropriate chemotherapeutic agents and provides evidence for individualized chemotherapy for breast cancer.
