Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 25, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

A PCR-based platform for microRNA expression profiling studies.

Xiaowei Wang1

  • 1Department of Radiation Oncology, Washington University School of Medicine, St. Louis, Missouri 63108, USA. xwang@radonc.wustl.edu

RNA (New York, N.Y.)
|February 17, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A MicroRNA Expression Signature as Prognostic Marker for Oropharyngeal Squamous Cell Carcinoma.

Journal of the National Cancer Institute·2020
Same author

Isoflurane activates AMP-activated protein kinase to inhibit proliferation, and promote apoptosis and autophagy in cervical carcinoma both <i>in vitro</i> and <i>in vivo</i>.

Journal of receptor and signal transduction research·2020
Same author

A prognostic gene expression signature for oropharyngeal squamous cell carcinoma.

EBioMedicine·2020
Same author

Branched α-helical peptides enhanced antitumor efficacy and selectivity.

Biomaterials science·2020
Same author

Robot-assisted versus laparoscopic surgery for rectal cancer: A systematic review and meta-analysis.

Journal of cancer research and therapeutics·2020
Same author

Investigations on the Optical Properties of InGaN/GaN Multiple Quantum Wells with Varying GaN Cap Layer Thickness.

Nanoscale research letters·2020
Same journal

Optimized tRNA structure-seq reveals robust tRNA secondary structures in <i>S. cerevisiae</i> under mild stress conditions.

RNA (New York, N.Y.)·2026
Same journal

SERIPH: A Two-Step Extraction Protocol for Selective Enrichment of Semi-Extractable RNAs.

RNA (New York, N.Y.)·2026
Same journal

Reduced Sensitivity to RNA Structural Differences Distinguishes Eukaryotic Pus4 from Bacterial TruB.

RNA (New York, N.Y.)·2026
Same journal

Puf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4.

RNA (New York, N.Y.)·2026
Same journal

RBM38 Regulates HORMAD1 Splicing to Enhances MEK Inhibitor Sensitivity in Breast Cancer.

RNA (New York, N.Y.)·2026
Same journal

EF-P Inhibits Ribosomal α-Hydroxy Acid Incorporation: Strategic tRNA Body Selection for Co-incorporating α-Hydroxy Acids and Nonproteinogenic Amino Acids into Depsipeptides.

RNA (New York, N.Y.)·2026
See all related articles

This study introduces a novel PCR-based assay for microRNA (miRNA) expression profiling. This cost-effective platform offers improved specificity and sensitivity over traditional microarray methods for disease research.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are small noncoding RNAs regulating biological processes.
  • Altered miRNA expression is linked to human diseases, including cancer.
  • Accurate miRNA profiling is crucial for understanding disease mechanisms.

Purpose of the Study:

  • To develop a novel, cost-effective PCR-based assay for miRNA expression profiling.
  • To overcome limitations associated with current microarray-based methods.
  • To enhance the specificity, sensitivity, and homogeneity of miRNA detection.

Main Methods:

  • Development of a PCR-based assay platform for miRNA expression analysis.
  • Design of miRNA assay primers using a novel bioinformatics algorithm.

More Related Videos

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

Related Experiment Videos

Last Updated: Jun 25, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

  • Incorporation of primer selection features for improved assay performance.
  • Experimental validation of the primer design algorithm and assay platform.

    • Main Results:

    • The novel bioinformatics algorithm ensures robust primer design for miRNA assays.
    • The PCR-based platform demonstrates improved specificity, sensitivity, and homogeneity.
    • The assay provides a cost-effective solution for miRNA expression measurement.
    • The platform is validated for measuring miRNA expression in diverse biological samples.

    Conclusions:

    • A new PCR-based assay platform offers a superior alternative for miRNA expression profiling.
    • The developed bioinformatics approach enhances the reliability of miRNA detection.
    • This technology facilitates low-cost, high-performance miRNA analysis for research and diagnostics.