Related Experiment Video
Updated: Jun 25, 2026

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System
Published on: October 22, 2020
Synergistic effects of cigarette smoke and saliva
Yalda Nozad-Mojaver1, Maysam Mirzaee, Abdullah Jafarzadeh
1Department of Oral Medicine, Rafsanjan University of Medical Sciences, Dental school, Rafsanjan, Iran. mojaver@farabi.tums.ac.ir
Objectives:
The aim of this study was to evaluate the cytotoxic effects of Cigarette Smoke on the human peripheral blood lymphocytes in the presence of stimulated or non-stimulated saliva in an in vitro model.
Methods And Materials:
Ten healthy volunteers in the age range of 21 to 29 were selected and samples of peripheral blood lymphocytes and saliva (whole and stimulated saliva) collected . Peripheral blood lymphocytes suspensions (PBS) were taken and exposed to 6 different media. Samples were collected from all media at both 20 and 80 minute time points. The survival rates of PBL were then determined at both 20 and 80 minute time points.
Results:
The cell survival rates following exposure to cigarette smoke (CS) in the presence of PBS supplemented with whole or stimulated saliva were significantly lower at 80 minutes when compared with the 20 minute rates (P<0.05).
Conclusion:
Tobacco effects were significantly increased in the presence of saliva especially stimulated saliva.
Related Concept Videos
Drugs Acting on Autonomic Ganglia: Stimulants
Ganglionic stimulants activate NM nicotinic receptors in autonomic ganglia, falling into two categories: nicotine mimetics [e.g., lobeline, dimethylpiperazine, tetramethylammonium] and muscarinic receptor agonists [e.g., muscarine, methacholine]. The first category's action is rapid and blocked by nicotinic receptor antagonists, while the second category's action is delayed and blocked by atropine-like agents. Nicotine, an alkaloid, affects the heart rate by stimulating sympathetic or...
Salivary Glands and Saliva
Combined Effects of Drugs: Synergism
Such synergistic combinations...
Stimulants
Cocaine can be administered via snorting, injection, or smoking. It primarily functions by blocking the reuptake of dopamine, resulting in a euphoric high characterized by an intense sensation of happiness and...
Chronic Obstructive Pulmonary Disease II: Emphysema
Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features
