Microbial degradation of cyclic peptides produced by bacteria

Bacterial strain, B-9, isolated from Lake Tsukui, Japan, and characterized as genus Sphingosinicella sp., possesses hydrolytic enzymes capable of degrading various toxic and non-toxic cyanobacterial cyclic peptides, such as microcystins, nodularin, microviridin, microcyclamide and aeruginopeptin. In this study, the degradation activities of the cell extract of B-9 against bacterial cyclic peptides, bacitracin, colistin, polymyxin, mikamycin, thiopeptin and WAP-8294A2, were investigated and the degradation products were analyzed using HPLC and liquid chromatography/ion trap tandem mass spectrometry (LC/ITMS). As a result of extensive experiments, it was confirmed that B-9 could also degrade these bacterial cyclic peptides by hydrolysis of their peptide or ester bonds, except for WAP-8294A2. These results indicated that the functions of the bacterium with its enzymes were further extended and offered the possibility of degrading other types of compounds.