Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optical Fourier Surfaces for Integrated Photonics.

ACS nano·2026

Same author

Electro-optic Modulation in Polycrystalline Barium Titanate Metasurfaces Enhanced by Poling.

ACS photonics·2026

Same author

High-efficiency multi-scale holographic volumetric 3D printing with a phase light modulator.

Light, science & applications·2026

Same author

Thin film lithium niobate on sapphire for integrated mid-infrared modulator.

Nature communications·2026

Same author

Temporal variance mapping with machine learning for label-free 3D chromatin imaging using optical interferometric microscopy.

Biomedical optics express·2026

Same author

Anode-Free Batteries: Pioneering Energy Storage Revolution.

Chemical reviews·2026

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Harmonic Nanoparticles for Regenerative Research

Harmonic Nanoparticles for Regenerative Research

Published on: May 1, 2014

Three-dimensional harmonic holographic microcopy using nanoparticles as probes for cell imaging.

Chia-Lung Hsieh¹, Rachel Grange, Ye Pu

¹School of Engineering, EPFL, Station 17, 1015 Lausanne, Switzerland. chia-lung.hsieh@epfl.ch

|February 17, 2009

Summary

Researchers developed novel barium titanate (BaTiO3) nanocrystals for advanced bioimaging. These second harmonic generation (SHG) markers offer stable, high-resolution 3D imaging in cells without scanning.

More Related Videos

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Related Experiment Videos

Last Updated: Jun 25, 2026

Harmonic Nanoparticles for Regenerative Research

Harmonic Nanoparticles for Regenerative Research

Published on: May 1, 2014

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Area of Science:

Biomedical Optics
Materials Science
Nanotechnology

Background:

Luminescent markers are crucial for life science imaging, providing signal-to-background contrast.
Existing markers have limitations, necessitating the development of new contrast agents.

Purpose of the Study:

To introduce and characterize barium titanate (BaTiO3) nanocrystals as novel luminescent markers.
To demonstrate the application of these BaTiO3 nanocrystals for high-resolution 3D imaging in biological systems.

Main Methods:

Synthesized monodispersed BaTiO3 nanocrystals (90 nm) coated with amine groups for colloidal suspension.
Characterized second harmonic generation (SHG) efficiency experimentally and theoretically.
Developed a harmonic holographic (H(2)) microscope to record digital holograms at the second harmonic frequency for 3D imaging.

Main Results:

Achieved stable, non-saturating, coherent SHG signals from BaTiO3 nanocrystals with femtosecond response.
Demonstrated successful 3D imaging of BaTiO3 nanoparticle distributions within mammalian cells using the H(2) microscope.
Captured high-resolution cellular distributions of the SHG markers.

Conclusions:

BaTiO3 nanocrystals are effective and versatile luminescent markers for bioimaging applications.
The H(2) microscope enables label-free, high-resolution 3D imaging of SHG nanoparticles in cells.
This technology offers a new avenue for advanced cellular imaging and diagnostics.