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Identification and characterization of a novel cytoskeleton-associated pp60src substrate
H Wu1, A B Reynolds, S B Kanner
1Department of Microbiology, Health Sciences Center, Charlottesville, Virginia 22908.
Molecular and Cellular Biology
|October 1, 1991
Summary
Src oncogene transformation alters the location of p80/85 proteins in cells. These proteins associate with F-actin, suggesting a role in cell structure organization.
Area of Science:
- Cell Biology
- Molecular Biology
- Oncogenesis
Background:
- The src oncogene is implicated in cellular transformation.
- Tyrosine phosphorylation of specific proteins is altered during transformation.
- The subcellular localization of proteins can change upon oncogenic transformation.
Purpose of the Study:
- To investigate the changes in p80/85 protein localization during src-mediated cellular transformation.
- To elucidate the structural features and potential functions of p80/85 proteins.
Main Methods:
- Immunostaining with monoclonal antibodies to detect p80/85 protein.
- Subcellular localization analysis using microscopy.
- Sequence analysis of cDNA clones encoding p80/85 proteins.
Main Results:
- Src oncogene transformation increases tyrosine phosphorylation of p80/85 proteins.
- p80/85 proteins exhibit altered subcellular localization, associating with F-actin in transformed cells.
- p80/85 proteins possess a multidomain structure including tandem repeats and an SH3 domain.
Conclusions:
- p80/85 proteins are dynamically regulated during src-mediated transformation.
- The association of p80/85 with F-actin suggests a role in cytoskeletal organization.
- These findings provide insights into the molecular mechanisms of oncogenic transformation and cell structure regulation.