Adding new dimensions to laser-scanning fluorescence microscopy
Related Experiment Video
Updated: Jun 25, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
1Department of Chemistry, Indian Institute of Technology Kanpur, UP 208016, India.
Abstract:
We describe a novel method of optical imaging by exploiting simple ideas borrowed from pulsed optics. We show that the use of ultrafast pulsed one-photon excitation in laser-scanning fluorescence microscopy dramatically brings together several advantages offered by two widely used present day microscopic techniques, confocal and multi-photon fluorescence microscopy. The method appears as a novel tool in the context of laser-scanning fluorescence microscopy by having a 'built-in' 3D spatial resolution.
Related Concept Videos
Confocal Fluorescence Microscopy
Super-resolution Fluorescence Microscopy
Three-Dimensional Microscopy in Microbiology
Two-Dimensional Microscopy in Microbiology

