Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy of Collagen Dressing for Scald Burn Among the Paediatric Population.

Annals of burns and fire disasters·2026
Same author

Lactation stages modulate the hematological, serum biochemical, and endocrinological profiles and oxidative stress markers in crossbred cows under tropical humid island ecosystem of Andaman and Nicobar Islands.

Tropical animal health and production·2023
Same author

Bone mineral density, vertebral fractures and trabecular bone score in primary ovarian insufficiency.

Journal of endocrinological investigation·2023
Same author

Flaxseed oil modulates testicular biometrics, hormone, libido, antioxidant and semen profiles in endangered Teressa goat of Andaman and Nicobar Islands.

Reproductive biology·2023
Same author

Daily and seasonal rhythmic secretary pattern of endocrinological profiles in mithun bull.

Theriogenology·2021
Same author

Slow release exogenous melatonin modulates scrotal circumference and testicular parameters, libido, endocrinological profiles and antioxidant and oxidative stress profiles in mithun.

Theriogenology·2020
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

Related Experiment Video

Updated: Jun 25, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Adding new dimensions to laser-scanning fluorescence microscopy.

A K De1, D Goswami

  • 1Department of Chemistry, Indian Institute of Technology Kanpur, UP 208016, India.

Journal of Microscopy
|February 18, 2009
PubMed
Summary
This summary is machine-generated.

A new optical imaging method uses ultrafast pulsed one-photon excitation in laser-scanning fluorescence microscopy. This technique combines benefits of confocal and multi-photon microscopy for built-in 3D resolution.

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
10:53

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

Related Experiment Videos

Last Updated: Jun 25, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
10:53

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

Area of Science:

  • Optics and Photonics
  • Microscopy
  • Biomedical Imaging

Background:

  • Confocal and multi-photon fluorescence microscopy are widely used techniques.
  • Each technique has limitations in resolution and imaging depth.
  • A need exists for advanced microscopy methods offering improved performance.

Purpose of the Study:

  • To introduce a novel optical imaging method.
  • To leverage ultrafast pulsed optics for enhanced fluorescence microscopy.
  • To integrate advantages of confocal and multi-photon microscopy.

Main Methods:

  • Development of a laser-scanning fluorescence microscopy technique.
  • Utilizing ultrafast pulsed one-photon excitation.
  • Exploiting principles from pulsed optics.

Main Results:

  • The novel method demonstrates significant advantages over existing techniques.
  • Achieved 'built-in' 3D spatial resolution.
  • Successfully combined benefits of confocal and multi-photon microscopy.

Conclusions:

  • The described method represents a novel tool for laser-scanning fluorescence microscopy.
  • Offers a unique approach to achieving high-resolution 3D imaging.
  • Potential to advance various fields requiring detailed microscopic visualization.