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Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...

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Related Experiment Video

Updated: Jun 25, 2026

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
07:02

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Published on: December 16, 2021

p53 methylation--the Arg-ument is clear.

Stephen T Durant1, Er Chieh Cho, Nicholas B La Thangue

  • 1Laboratory of Cancer Biology, Department of Clinical Pharmacology, Medical Sciences Division, University of Oxford, Oxford, UK.

Cell Cycle (Georgetown, Tex.)
|February 18, 2009
PubMed
Summary

The tumor suppressor p53 is crucial for preventing cancer. Arginine methylation of p53 by PRMT5, a newly identified modification, affects its function after DNA damage.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • The tumor suppressor p53 is essential for preventing human cancer, with its inactivation common in tumors.
  • p53 functions primarily as a sequence-specific transcription factor, regulating genes involved in apoptosis and cell cycle arrest.
  • p53 activity is modulated by post-translational modifications like phosphorylation and methylation.

Purpose of the Study:

  • To investigate the role of arginine methylation, specifically mediated by PRMT5, as a novel post-translational modification of p53.
  • To understand how DNA damage influences p53 arginine methylation and its impact on p53 function.

Main Methods:

  • Analysis of p53 post-translational modifications.
  • Investigating the enzymatic activity of PRMT5 on p53.

More Related Videos

In vitro Methylation Assay to Study Protein Arginine Methylation
10:01

In vitro Methylation Assay to Study Protein Arginine Methylation

Published on: October 5, 2014

Related Experiment Videos

Last Updated: Jun 25, 2026

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
07:02

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Published on: December 16, 2021

In vitro Methylation Assay to Study Protein Arginine Methylation
10:01

In vitro Methylation Assay to Study Protein Arginine Methylation

Published on: October 5, 2014

  • Assessing the functional consequences of p53 arginine methylation on gene regulation and cellular responses to DNA damage.

    • Main Results:

    • Arginine methylation of p53 by PRMT5 is identified as a significant post-translational modification.
    • DNA damage induces p53 arginine methylation.
    • This modification alters the biochemical properties and functional outcomes of the p53 response.

    Conclusions:

    • Arginine methylation by PRMT5 is a critical regulatory mechanism for p53 activity.
    • Understanding p53 arginine methylation provides new insights into cancer suppression pathways.
    • Targeting PRMT5 or modulating p53 methylation could offer novel therapeutic strategies for cancer.