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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jun 25, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

Technical analysis of cDNA microarrays.

Cinda P Scott1, Jeff VanWye, M Danielle McDonald

  • 1Department of Marine Biology and Fisheries, Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, Florida, United States of America.

Plos One
|February 18, 2009
PubMed
Summary
This summary is machine-generated.

Microarray gene expression measurements are precise and quantitative, with minimal technical variation from standard procedures. Most variation observed is not biological noise, making microarrays reliable for research.

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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

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Performing Custom MicroRNA Microarray Experiments
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Performing Custom MicroRNA Microarray Experiments

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Last Updated: Jun 25, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Performing Custom MicroRNA Microarray Experiments
07:04

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Gene expression varies significantly within and between populations.
  • Technical variations in RNA isolation, labeling, and hybridization can confound microarray accuracy.
  • Understanding sources of variation is crucial for reliable gene expression analysis.

Purpose of the Study:

  • To assess the linearity between mRNA concentration and microarray fluorescent signal.
  • To quantify the impact of technical procedures on gene expression measurements.
  • To differentiate between technical and biological variation in microarray data.

Main Methods:

  • Analysis of the relationship between mRNA amount and fluorescent signal in microarray hybridizations.
  • Evaluation of variation introduced by RNA isolation, labeling, and hybridization steps.
  • Time-course measurement of mRNA expression in five individuals over six weeks to assess technical and biological variation.

Main Results:

  • Microarray fluorescent signal is a linear function of mRNA concentration across a wide range.
  • Individual RNA isolation, labeling, or hybridization steps do not introduce significant variation.
  • Single or double rounds of RNA amplification for labeling have minor, avoidable effects on approximately 10% of genes.

Conclusions:

  • Microarray analysis using standard laboratory protocols provides precise and quantitative gene expression data.
  • Significant random biological noise does not substantially impact microarray measurements.
  • Microarrays are reliable tools for studying gene expression variation when technical factors are controlled.