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Surface components involved in virally mediated membrane changes.

K J Micklem, C A Pasternak

    The Biochemical Journal
    |February 15, 1977
    PubMed
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    Sendai virus alters cell membrane permeability by increasing calcium ion (Ca2+) exchange, not through enzymes or anesthetics. This calcium exchange is an early event following virus attachment.

    Area of Science:

    • Cell biology
    • Virology
    • Membrane biophysics

    Background:

    • Viral infections can alter host cell membrane properties.
    • Understanding virus-host interactions at the membrane level is crucial for virology.
    • Calcium ions play significant roles in cellular processes and membrane dynamics.

    Purpose of the Study:

    • To investigate the mechanisms by which viruses, specifically Sendai virus, induce changes in cell membrane permeability.
    • To determine if enzymatic activity or local anesthetics are involved in virus-mediated permeability changes.
    • To elucidate the role of membrane-bound calcium ions in the early stages of viral attachment and its effect on cell permeability.

    Main Methods:

    • Investigating the effects of neuraminidase and local anesthetics on virus-induced permeability.

    Related Experiment Videos

  • Measuring the exchange rate of membrane-bound calcium ions in cultured cells upon viral exposure.
  • Comparing the temperature dependence and concanavalin A sensitivity of permeability changes and calcium exchange.

    • Main Results:

    • Viral-mediated permeability changes were not caused by enzymes or local anesthetics like benzyl alcohol or dibucaine.
    • Sendai virus infection led to an increased exchange rate of membrane-bound calcium ions (Ca2+).
    • The temperature dependence and concanavalin A sensitivity of both the permeability change and Ca2+ exchange were found to be similar.

    Conclusions:

    • The observed changes in cell membrane permeability induced by Sendai virus are not mediated by enzymatic activity or anesthetic-like mechanisms.
    • An increased exchange rate of membrane-bound calcium ions is a key event triggered by Sendai virus attachment.
    • The findings suggest that altered calcium ion dynamics are an early and specific response of cultured cells to Sendai virus, impacting membrane permeability.