Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 25, 2026

High-Throughput Measurement and Classification of Organic P in Environmental Samples
08:58

High-Throughput Measurement and Classification of Organic P in Environmental Samples

Published on: June 8, 2011

Alkaline phosphatase-polyresorcinol complex: characterization and application to seed coating.

María C Pilar1, Natividad Ortega, Manuel Perez-Mateos

  • 1Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Banuelos, s/n. 09001 Burgos, Spain.

Journal of Agricultural and Food Chemistry
|February 19, 2009
PubMed
Summary

Immobilized alkaline phosphatase (EC 3.1.3.1) using polyresorcinol enhances soil phosphorus availability and plant growth. This enzyme complex shows improved stability and activity in soil applications, benefiting barley cultivation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Innovative enzyme-based seed coatings for wheat: Impacts on plant growth, P and N uptake, and rhizosphere enzyme profile.

New biotechnology·2026
Same author

Dual-range colorimetric protein quantification in whey and WPC using a smart polymeric film and RGB imaging.

Food chemistry·2026
Same author

Immobilization of Rhodococcus by encapsulation and entrapment: a green solution to bitter citrus by-products.

Applied microbiology and biotechnology·2023
Same author

Detection of Hazelnut and Almond Adulteration in Olive Oil: An Approach by qPCR.

Molecules (Basel, Switzerland)·2023
Same author

Microencapsulation of a Commercial Food-Grade Protease by Spray Drying in Cross-Linked Chitosan Particles.

Foods (Basel, Switzerland)·2022
Same author

Kinetic Modeling, Thermodynamic Approach and Molecular Dynamics Simulation of Thermal Inactivation of Lipases from <i>Burkholderia cepacia</i> and <i>Rhizomucor miehei</i>.

International journal of molecular sciences·2022

Area of Science:

  • Biochemistry
  • Enzyme Immobilization
  • Environmental Biotechnology

Background:

  • Alkaline phosphatase (ALP) is crucial for phosphorus cycling.
  • Enzyme immobilization enhances stability and reusability.
  • Escherichia coli ATCC27257 provides a source of alkaline phosphatase.

Purpose of the Study:

  • To immobilize alkaline phosphatase from E. coli ATCC27257 using copolymerization with resorcinol.
  • To evaluate the biochemical properties and stability of the immobilized enzyme.
  • To assess the efficacy of immobilized ALP in soil and its impact on plant growth.

Main Methods:

  • Enzyme immobilization via copolymerization with resorcinol.
  • Characterization of pH, temperature profiles, and kinetic parameters (Km, Ki).

More Related Videos

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
08:51

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification

Published on: November 19, 2018

Related Experiment Videos

Last Updated: Jun 25, 2026

High-Throughput Measurement and Classification of Organic P in Environmental Samples
08:58

High-Throughput Measurement and Classification of Organic P in Environmental Samples

Published on: June 8, 2011

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
08:51

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification

Published on: November 19, 2018

  • Assessment of thermal, storage, and soil stability.
  • Evaluation of effects on barley seed rhizosphere activity, soil inorganic phosphorus, and plant biomass.

    • Main Results:

    • The phosphatase-polyresorcinol complex retained 74% of original activity.
    • Immobilized ALP exhibited similar pH/temperature profiles but altered kinetic parameters (higher Km, Ki) compared to free enzyme.
    • Enhanced thermal, storage, and soil stability for the immobilized enzyme.
    • Increased rhizosphere phosphatase activity and soil inorganic phosphorus levels with immobilized enzyme-coated barley seeds.
    • Positive impact on barley biomass and shoot inorganic phosphorus concentration.

    Conclusions:

    • Enzyme immobilization using polyresorcinol is an effective strategy to enhance alkaline phosphatase stability and functionality.
    • The phosphatase-polyresorcinol complex shows significant potential for improving soil phosphorus availability and promoting plant growth.
    • This approach offers a promising avenue for agricultural applications, particularly in enhancing crop yield and nutrient uptake.