Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Digital twins in medicine.

Nature computational science·2024

Same author

Mercury and selenium concentrations in lanugo of free-ranging California sea lions in the southern Gulf of California, Mexico.

Marine pollution bulletin·2023

Same author

First Observation of Cyclotron Radiation from MeV-Scale e^{±} following Nuclear β Decay.

Physical review letters·2023

Same author

Church Support among African American and Black Caribbean Adolescents.

Journal of child and family studies·2020

Same author

Substantial and sustained reduction in under-5 mortality, diarrhea, and pneumonia in Oshikhandass, Pakistan: evidence from two longitudinal cohort studies 15 years apart.

BMC public health·2020

Same author

A 192-heme electron transfer network in the hydrazine dehydrogenase complex.

Science advances·2019

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Published on: October 4, 2013

Dynamic analysis of MAPK signaling using a high-throughput microfluidic single-cell imaging platform.

R J Taylor¹, D Falconnet, A Niemistö

¹Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.

Proceedings of the National Academy of Sciences of the United States of America

|February 19, 2009

Summary

Researchers developed a microfluidic platform for high-throughput single-cell analysis. This system enables detailed studies of cellular responses to genetic changes and dynamic environments, revealing new insights into cell decision-making.

More Related Videos

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

Related Experiment Videos

Last Updated: Jun 25, 2026

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Published on: October 4, 2013

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

Area of Science:

Cellular biology
Systems biology
Biophysics

Background:

Cells utilize complex biomolecular networks to respond to environmental cues.
Understanding emergent network properties requires analyzing cellular responses under diverse conditions.
Scalable analysis of cell behavior under dynamic conditions has been a technological challenge.

Purpose of the Study:

To develop a high-throughput platform for single-cell analysis of network responses.
To investigate cellular decision-making under combined genetic perturbations and time-varying stimuli.
To explore the mating pheromone response in Saccharomyces cerevisiae.

Main Methods:

Developed a microfluidic imaging platform with programmable on-chip mixing and perfusion.
Utilized 2,048 microfluidic cell traps for high-throughput live-cell imaging.
Performed 256 simultaneous time-lapse experiments analyzing 8 genotypes over 12 hours with 32 stimulation sequences.

Main Results:

Conducted over 3,000 live-cell imaging experiments across 12 devices.
Analyzed 11 deletion mutants, identifying distinct thresholds for morphological switching.
Observed novel dynamic phenotypes, including stimulus-frequency-dependent signaling in specific mutants (kss1Δ, fus3Δ, msg5Δ, ptp2Δ), suggesting roles in filtering and memory.

Conclusions:

The developed platform enables scalable, time-resolved studies of single-cell responses.
Dynamic cellular phenotypes and network memory mechanisms were revealed under varying conditions.
This technology provides a powerful tool for systems-level investigations of cellular decision-making.