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Using gene expression to predict the secretome of differentiating human preadipocytes
D M Mutch1, C Rouault, M Keophiphath
1INSERM, UMRS U872 (Eq 7) Nutriomique, Centre de Recherche des Cordeliers, Paris, France. dmutch@uoguelph.ca
International Journal of Obesity (2005)
|February 19, 2009
Summary
This study identified novel adipose tissue secretome genes during human preadipocyte differentiation. Matrix Gla Protein (MGP) and Inhibin beta B (INHBB) were confirmed as new adipokines.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Adipose tissue secretome characterization is crucial for understanding metabolic regulation.
- Differentiating preadipocytes release signaling molecules that influence systemic metabolism.
- Global gene expression profiling offers a comprehensive approach to identify secreted proteins.
Purpose of the Study:
- To identify and characterize the secretome of differentiating human preadipocytes.
- To discover novel secreted proteins involved in adipogenesis.
- To validate candidate secretome genes at the protein level.
Main Methods:
- Global gene expression profiling using microarrays in differentiating human preadipocytes.
- Bioinformatic algorithms to predict genes encoding secreted proteins.
- Validation of protein secretion using Western blots and ELISA assays.
- Gene expression analysis in adipocytes and stromal vascular fraction (SVF) from obese subjects.
Main Results:
- 33 significantly differentially expressed genes encoding secreted proteins were identified during adipogenesis.
- 18 novel candidate adipose tissue secretome genes were discovered.
- Matrix Gla Protein (MGP) and Inhibin beta B (INHBB) were identified as novel adipokines, with MGP showing a 30-fold upregulation.
- Most novel secretome genes were more highly expressed in the SVF compared to adipocytes in obese subjects.
Conclusions:
- Gene expression profiling combined with bioinformatic analysis is effective for defining the adipocyte secretome.
- Novel secretome genes, particularly those in coagulation and fibrinolytic systems, were identified.
- MGP and INHBB are proposed as new adipokines requiring further functional investigation.
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