Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct visualization of native GSDMD pores reveals lipid-driven stabilization during pyroptosis.

Science advances·2026
Same author

VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair.

Nature communications·2026
Same author

Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets.

Nature communications·2026
Same author

Metal-Coordinated His-Tag Functionalization of Polymeric Nanogels for Therapeutic Applications.

ACS applied nano materials·2026
Same author

A Modular Toolkit for Nanoscale Interrogation of Multiprotein Assemblies Inside Living Cells.

ACS nano·2026
Same author

Hour-scale single-molecule imaging reveals dynamic assembly of the Wnt co-receptors LRP6 and ROR2 into common signalosomes.

Science signaling·2026

Related Experiment Video

Updated: Jun 25, 2026

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

Optical force sensor array in a microfluidic device based on holographic optical tweezers.

Kai Uhrig1, Rainer Kurre, Christian Schmitz

  • 1Max-Planck-Institute for Metals Research, Department of New Materials and Biosystems, Heisenbergstr. 3, D-70569 Stuttgart, Germany.

Lab on a Chip
|February 19, 2009
PubMed
Summary

Holographic optical tweezers integrated with microfluidics enable precise force measurements. This novel setup allows detailed investigation of chemo-mechanical processes in biomimetic networks.

More Related Videos

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution
09:31

Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution

Published on: May 27, 2013

Related Experiment Videos

Last Updated: Jun 25, 2026

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution
09:31

Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution

Published on: May 27, 2013

Area of Science:

  • Biophysics
  • Microfluidics
  • Optical manipulation

Background:

  • Holographic optical tweezers (HOT) offer advanced control over microparticle manipulation and force measurement.
  • Integrating HOT with microfluidics and microscopy enhances experimental capabilities for biological studies.

Purpose of the Study:

  • To develop and demonstrate an integrated holographic optical tweezers and microfluidics platform.
  • To probe chemo-mechanical properties of cellular or subcellular structures with high spatial, chemical, and visual control.
  • To showcase the platform's utility as a reconfigurable force sensor array.

Main Methods:

  • Combined holographic optical tweezers with a fluorescence microscope and a multi-channel microfluidic device.
  • Utilized a high-speed camera for simultaneous trap calibration via Boltzmann statistics or power spectrum density.
  • Constructed a biomimetic actin network on trapped polystyrene microspheres within the microfluidic chamber.

Main Results:

  • Achieved precise control over the microenvironment for probing chemo-mechanical properties.
  • Observed the buildup of mechanical tension in an actin network during crosslinking with Mg(2+) ions.
  • Demonstrated piconewton force resolution for investigating chemo-mechanical processes.

Conclusions:

  • The integrated HOT-microfluidics platform provides a versatile tool for studying complex chemo-mechanical interactions.
  • This system enables detailed analysis of biomimetic networks and cellular structures.
  • The platform serves as a reconfigurable force sensor array for advanced biophysical research.