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Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
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Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Published on: May 21, 2018

Structural characterization of lipopeptides from Brevibacillus brevis HOB1.

Ji Wang1, Namir I A Haddad, Shi-Zhong Yang

  • 1State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, China.

Applied Biochemistry and Biotechnology
|February 19, 2009
PubMed
Summary

Brevibacillus brevis HOB1, isolated from oil field water, produces novel surfactin isoforms. These lipopeptides demonstrate significant biosurfactant properties, expanding the known surfactin-producing species.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Organic Chemistry

Background:

  • Lipopeptides are amphipathic compounds with diverse biological activities.
  • Surfactin, a well-known lipopeptide biosurfactant, is produced by Bacillus subtilis.
  • The exploration of microbial diversity in extreme environments can yield novel bioactive compounds.

Purpose of the Study:

  • To isolate and characterize lipopeptides produced by Brevibacillus brevis HOB1 from an oil field.
  • To elucidate the chemical structures and identify the isoforms of the produced lipopeptides.
  • To evaluate the biosurfactant activity of the identified lipopeptides.

Main Methods:

  • Bacterial isolation from oil field formation water.
  • Reversed-phase high-performance liquid chromatography (HPLC) for lipopeptide separation.
  • Electrospray ionization mass spectrometry (ESI-MS), gas chromatography-mass spectrometry (GC/MS), and ESI-MS/MS for structural elucidation.

Main Results:

  • Nine lipopeptide peaks were separated from Br. brevis HOB1 culture.
  • All lipopeptides shared a common peptide moiety (Asp, Glu, Val, Leu in 1:1:1:4 ratio) and C(13)-C(15) beta-hydroxy fatty acid lipid tails.
  • Fraction 1 was identified as surfactin, and other fractions were proposed as surfactin isoforms. Fraction 4 (C(15) surfactin) showed strong surface activity (26.8 mN/m) at a low critical micelle concentration (CMC) of 9 x 10(-6) M.

Conclusions:

  • Brevibacillus brevis HOB1 is a newly identified species capable of producing surfactin and its isoforms.
  • The identified lipopeptides possess potent biosurfactant properties.
  • This discovery expands the known microbial sources of powerful biosurfactants.