HPLC analysis of platinum cytostatics

Area of Science:

• Analytical Chemistry
• Pharmacology
• Clinical Chemistry

Background:

• Platinum-based chemotherapy drugs, including cisplatin and carboplatin, are crucial in cancer treatment.
• Understanding their stability and behavior in biological matrices is essential for effective therapeutic monitoring.
• Accurate quantification in clinical samples requires robust analytical methods.

Purpose of the Study:

• To separate and analyze platinum cytostatics (cisplatin, carboplatin, oxo-carboplatin) using HPLC-DAD.
• To investigate the stability of these drugs under various conditions (chloride, pH, temperature, time).
• To develop and validate a method for carboplatin extraction and quantification in serum samples.

Main Methods:

• High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD) for separation and detection.
• Study of drug stability through aquation reaction kinetics.
• Solid-phase extraction (SPE) for sample preparation of clinical samples.

Main Results:

• Successful separation and stability assessment of cisplatin, carboplatin, and oxo-carboplatin.
• Determination of rate constants for aquation reactions under varying conditions.
• Demonstrated high extraction recoveries and low relative standard deviations for carboplatin in serum using SPE.

Conclusions:

• HPLC-DAD is an effective technique for analyzing platinum cytostatics and their stability.
• The characterized stability provides valuable data for understanding drug behavior in vivo.
• Solid-phase extraction is a recommended and reliable method for clinical analysis of carboplatin in serum.