HPLC analysis of platinum cytostatics

High performance liquid chromatography (HPLC) with diode array detector (DAD) was applied for the separation of platinum cytostatics (cisplatin, carboplatin and oxo-carboplatin). Their stabilities were studied in water and the influence of chloride anions, pH, temperature and time was discussed and rate constants of the aquation reactions at different conditions were calculated. Ligand exchange reactions cisplatin----carboplatin were evaluated using HPLC-DAD system. Detection limits for all studied cytostatics were determined and extraction recoveries for carboplatin in clinical samples (serum) were demonstrated for a wide range of concentrations together with the relative standard deviations. The solid-phase extraction procedure was recommended for clinical sample analysis represented by a model mixture and real patients' samples.