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Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
Published on: June 24, 2018
Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates
Nicoletta Bivi1, Jessica Z Bereszczak, Milena Romanello
1Department of Biomedical Sciences and Technologies, University of Udine, Udine, Italy, Shimadzu Research Laboratory (Europe), Manchester, UK.
Journal of Proteome Research
|February 20, 2009
Summary
Risedronate treatment upregulates zinc-proteins in osteocytes, affecting proteins involved in cytoskeleton, stress, and metabolism. This research clarifies how bisphosphonates protect osteocytes from apoptosis.
Area of Science:
- Molecular Biology
- Cell Biology
- Pharmacology
Background:
- Osteocytes are crucial bone cells.
- Bisphosphonates, like Risedronate, are used to treat bone diseases.
- Understanding their molecular mechanisms is important.
Purpose of the Study:
- To investigate the molecular effects of Risedronate on osteocytes.
- To identify specific proteins and pathways affected by Risedronate treatment.
Main Methods:
- High-throughput screening of differential messenger RNAs (mRNAs).
- Mass spectrometry (MS) for proteomic analysis of osteocytes.
- Gel imaging and GLaD post-digestion isotopic labeling for data validation.
Main Results:
- Risedronate treatment significantly upregulated mRNAs encoding zinc-proteins.
- Mass spectrometry identified 84 proteins in osteocytes.
- Risedronate altered the expression of 10 proteins related to cytoskeleton, stress response, and metabolism.
Conclusions:
- The study provides a molecular basis for Risedronate's antiapoptotic effects on osteocytes.
- Findings highlight the role of zinc-proteins and specific cellular pathways in bisphosphonate action.
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