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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
Published on: July 17, 2018
Functional implications of CD34 expression in human adipose-derived stem/progenitor cells
Hirotaka Suga1, Daisuke Matsumoto, Hitomi Eto
1Department of Plastic Surgery, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Stem Cells and Development
|February 20, 2009
Summary
CD34 expression in human adipose-derived stem cells (hASCs) impacts their function. CD34+ cells are more proliferative, while CD34- cells differentiate better, suggesting CD34 marks stemness and early lineage commitment.
Area of Science:
- Stem cell biology
- Cellular differentiation
- Tissue engineering
Background:
- CD34 is a common marker for adipose-derived stem/stromal/progenitor cells (ASCs).
- CD34 expression decreases in human ASCs (hASCs) during culture, but its functional significance is not fully understood.
Purpose of the Study:
- To investigate the functional differences between CD34-positive (CD34+) and CD34-negative (CD34-) hASCs.
- To elucidate the role of CD34 expression in hASC stemness, proliferation, differentiation, and angiogenesis-related gene expression.
Main Methods:
- Short-term cultured hASCs were sorted into CD34+ and CD34- fractions.
- Biological functions including proliferation, colony formation, differentiation, migration, and capillary formation were compared.
- Expression levels of endothelial progenitor and pericyte markers were analyzed.
Main Results:
- CD34+ hASCs exhibited higher proliferation and colony-forming abilities.
- CD34- hASCs demonstrated enhanced differentiation into adipogenic and osteogenic lineages.
- Endothelial progenitor markers were higher in CD34+ cells, while pericyte markers were higher in CD34- cells.
- Migration and capillary formation abilities were similar between both fractions.
Conclusions:
- CD34 expression in hASCs correlates with replicative capacity, differentiation potential, and stemness.
- Loss of CD34 expression may indicate commitment and differentiation towards specific lineages like adipose, bone, or smooth muscle.
