Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.

Oxygen Requirements and Growth Patterns

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...

Composition of Blood Plasma

Composition of Blood Plasma

Blood plasma is a fluid that contains approximately 92% water and 8% solutes. The solutes include various types of proteins, which constitute about 7% of the total solutes in the plasma. The high-molecular-weight proteins—albumins, globulins, and fibrinogen—are essential to plasma function. Albumins, making up about 60% of the plasma proteins, maintain the osmotic balance within blood vessels by preventing excessive water leakage. Additionally, albumins serve as carrier proteins, binding to...

Chemical Agents for Microbial Control

Chemical Agents for Microbial Control

Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...

Bioreactor Controls-II

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

Antimicrobial Effectiveness

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of Influenza Vaccine on the Immune Responses to SARS-CoV-2 Vaccination.

Vaccines·2024

Same author

Search for Dark-Matter-Nucleon Interactions via Migdal Effect with DarkSide-50.

Physical review letters·2023

Same author

Search for Dark Matter Particle Interactions with Electron Final States with DarkSide-50.

Physical review letters·2023

Same author

Constraints on the K_{S}^{0}→μ^{+}μ^{-} Branching Fraction.

Physical review letters·2020

Same author

Observation of Enhanced Double Parton Scattering in Proton-Lead Collisions at sqrt[s_{NN}]=8.16 TeV.

Physical review letters·2020

Same author

Safety, pitfalls, and misunderstandings about the use of ozone therapy as a regenerative medicine tool. A narrative review.

Journal of biological regulators and homeostatic agents·2020

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

How much ozone bactericidal activity is compromised by plasma components?

S Burgassi¹, I Zanardi, V Travagli

¹Dipartimento di Fisiopatologia, Medicina Sperimentale e Sanità Pubblica, Università degli Studi di Siena, Italy.

Journal of Applied Microbiology

|February 20, 2009

Summary

This summary is machine-generated.

Ozone effectively kills bacteria in saline, but human plasma significantly reduces its effectiveness. Topical ozone remains valuable for skin and mucosal infections despite limited in vivo systemic effects.

More Related Videos

Implementation of a Hyperbolic Vortex Plasma Reactor for the Removal of Micropollutants in Water

Implementation of a Hyperbolic Vortex Plasma Reactor for the Removal of Micropollutants in Water

Published on: July 25, 2025

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

Published on: November 30, 2017

Related Experiment Videos

Last Updated: Jun 25, 2026

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

Implementation of a Hyperbolic Vortex Plasma Reactor for the Removal of Micropollutants in Water

Implementation of a Hyperbolic Vortex Plasma Reactor for the Removal of Micropollutants in Water

Published on: July 25, 2025

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

Published on: November 30, 2017

Area of Science:

Microbiology
Biochemistry
Ozone Therapy

Background:

Ozone is recognized for its potent antimicrobial properties.
Understanding ozone's efficacy in biological environments is crucial for therapeutic applications.

Purpose of the Study:

To evaluate the bactericidal effects of ozone, hydrogen peroxide, and 4-hydroxynonenal.
To investigate the influence of human plasma on ozone's antimicrobial activity.

Main Methods:

Bacteria (Staphylococcus aureus, MRSA, Pseudomonas aeruginosa) were exposed to varying concentrations of ozone (gas or dissolved in saline).
The impact of human plasma, hydrogen peroxide, and 4-hydroxynonenal on bacterial viability was assessed.

Main Results:

Ozone demonstrated potent bactericidal activity in protein-free saline.
As little as 5% human plasma inhibited ozone's bactericidal effect.
Hydrogen peroxide was less potent than ozone, and 4-hydroxynonenal showed inhibitory effects on specific bacteria.

Conclusions:

Ozone and its derivatives are effective bactericides, but antioxidants in human plasma neutralize their activity.
Systemic in vivo bactericidal effects of ozone are unlikely, but topical applications are beneficial for infections.