Comparative methods for the evaluation of protein adsorption

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Monitoring Protein Adsorption with Solid-state Nanopores
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Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

Eva Servoli1, Devid Maniglio, Maria Rosa Aguilar

  • 1Department of Materials Engineering and Industrial Technologies and INSTM Research Unit, University of Trento, via Mesiano 77, 38100 Trento, Italy. servolie@ing.unitn.it

Macromolecular Bioscience
|February 20, 2009
PubMed

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Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...