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Related Experiment Video

Updated: Jun 25, 2026

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
10:28

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Published on: June 30, 2016

Revisiting optical clearing with dimethyl sulfoxide (DMSO).

Albert K Bui1, R Anthony McClure, Jennell Chang

  • 1School of Biological Sciences, University of California, Irvine, California 92697, USA.

Lasers in Surgery and Medicine
|February 20, 2009
PubMed
Summary

Dimethyl sulfoxide (DMSO) reduces skin optical scattering, improving light penetration and subsurface imaging. This finding enhances molecular imaging studies by mitigating scattering effects.

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Last Updated: Jun 25, 2026

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
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Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging
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Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging

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Area of Science:

  • Biomedical Optics
  • Dermatology
  • Medical Imaging

Background:

  • Optical scattering in skin limits imaging depth and resolution.
  • Accurate disease progression and therapy response monitoring are hindered by scattering.
  • Need for methods to improve optical imaging in biological tissues.

Purpose of the Study:

  • To investigate the potential of dimethyl sulfoxide (DMSO) to reduce skin optical scattering.
  • To assess the impact of DMSO on light transmittance and microvasculature visualization in vivo.
  • To establish DMSO as a tool for enhancing molecular imaging in dermatology.

Main Methods:

  • Topical application of DMSO to skin samples.
  • Measurement of optical scattering reduction.
  • In vivo window chamber model to assess light transmittance.
  • Visualization of subsurface microvasculature.

Main Results:

  • Topical DMSO application resulted in a threefold reduction in skin optical scattering.
  • A threefold increase in light transmittance was observed through DMSO-treated skin.
  • Enhanced visualization of subsurface microvasculature was achieved.

Conclusions:

  • Dimethyl sulfoxide (DMSO) effectively reduces skin optical scattering.
  • DMSO improves light penetration, enabling better visualization of subsurface structures.
  • DMSO shows significant potential for enhancing molecular imaging studies in vivo.