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Updated: Jun 25, 2026

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
Published on: April 16, 2021
[Evaluation of the INNO-LiPA MYCOBACTERIA v2 for mycobacterial identification]
Shiomi Yoshida1, Katsuhiro Suzuki, Kazunari Tsuyuguchi
1Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan. dustin@kch.hosp.go.jp
Purpose:
Evaluation of the INNO-LiPA MYCOBACTERIA v2 (the INNO-LiPA assay) for mycobacterial identification.
Materials And Methods:
The laboratory identifications consisting of Cobas Amplicor systems, AccuProbe, and DDH, are commonly used to identify mycobacterial isolates in Japan. We compared the results between the INNO-LiPA assay and the common methods. A total of 122 clinical isolates from NHO Kinki-chuo Chest Medical Center from 1 February to 30 June 2006 were tested.
Results:
There was agreement between the INNO-LiPA assay and the common methods for 112 mycobacterium isolates. The six discordant isolates have showed same results between sequencings and the INNO-LiPA assay. The one M. fortuitum isolates was indicated correctness by DDH and the one M. intracellulare isolates was recognized by Cobas Amplicor systems and as MAC by AccuProbe MAC. Moreover, discrepant results between sequencings and mycobacterial identifications including the INNO-LiPA assay were 2 isolates (M. paraffinicum, M. mucogenicum variant type).
Conclusion:
The INNO-LiPA assay could provide rapid and correct identification results with clear-cut and easy interpretation.
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