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Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Antimutagenic efficacy of some natural compounds on cyclophosphamide-induced p53 alterations

Eman M Gouda1, Adel M Elbehairy, Magdy A Ghoneim

  • 1Biochemistry Department and Biotechnology Center, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt. emanmgouda@hotmail.com

Zeitschrift Fur Naturforschung. C, Journal of Biosciences
|February 21, 2009
PubMed

Insights

Natural antimutagens like chlorophyllin and curcumin show promise in preventing p53 gene mutations caused by the chemical carcinogen cyclophosphamide (CP). Chlorophyllin demonstrated a higher protective efficacy against these p53 alterations.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Toxicology

Background:

  • Mutations in the p53 tumor suppressor gene are linked to chemical carcinogens.
  • Natural antimutagens offer potential strategies for cancer risk reduction.
  • Assessing natural compounds' efficacy against p53 alterations is crucial for cancer prevention.

Purpose of the Study:

  • To evaluate the protective effects of curcumin and chlorophyllin against p53 gene mutations induced by cyclophosphamide (CP).
  • To investigate the modulation of p53 alterations by natural antimutagens in a rat model.

Main Methods:

  • Rats were pretreated with curcumin or chlorophyllin before CP injection.
  • DNA was isolated from liver samples for analysis.
  • Single-strand conformational polymorphism (SSCP) analysis was performed on PCR-amplified p53 gene regions (exons 5, 6, and 7).

Main Results:

  • Cyclophosphamide (CP) induced a mutation in exon 7 of the p53 gene, indicated by a shift in electrophoretic mobility.
  • Chlorophyllin pretreatment exhibited a significant protective efficacy of 80% against CP-induced p53 mutations.
  • Curcumin pretreatment showed a lower protective efficacy of 33.3%.

Conclusions:

  • Chlorophyllin is a potent natural antimutagen effective in preventing cyclophosphamide-induced p53 gene mutations.
  • Curcumin also offers some protection but to a lesser extent than chlorophyllin.
  • These findings support the potential of natural antimutagens in chemoprevention strategies against carcinogen-induced DNA damage.

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