Related Experiment Video
Updated: Jun 25, 2026

Establishment of a Murine Pulp Exposure Model with a Novel Mouth-Gag for Pulpitis Research
Published on: October 27, 2023
Effect of simulated pulpal microcirculation on intrapulpal temperature changes following application of heat on tooth
K Kodonas1, C Gogos, D Tziafas
1Department of Endodontology, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece. kkodonas@dent.auth.gr
Aim:
To evaluate ex vivo whether a simulated pulpal microcirculation inside a pulp chamber influenced intrapulpal temperature rise following application of heat on tooth surfaces.
Methodology:
An ex vivo model that allowed the circulation of 37 degrees C warm water inside the pulp chamber of an extracted human tooth was designed. The experimental model resembled pulpal microcirculation. After application of specific thermal stimuli for 30 s to the external surface of 15 maxillary central incisors, lateral incisors and canines, temperature changes were measured in the pulp chamber. The Greenhouse-Geisser and Bonferroni tests were used for analysis of the data. The level of significance was set at 0.05.
Results:
Significant differences were found in all three groups of teeth between temperature measurements with or without intrapulpal water flow. Additionally, temperature changes resulting from the application of different stimuli to the group of lateral incisors were significantly greater compared with the other groups of teeth (P < 0.05).
Conclusions:
The importance of the cooling effect of simulated pulp microcirculation in the thermal behaviour of the dentine was established. Thickness of tooth tissue influenced significantly pulp temperature rise ex vivo.
Related Concept Videos
Assessing Body Temperature - Temporal Artery
Step 1: Perform hand hygiene and don a fresh pair of gloves to prevent cross-infection and ensure patient safety.
Step 2: Explain the procedure to the patient to establish trust. Clear communication establishes trust with the patient, ensures they understand what to expect, promotes cooperation, and enhances comfort during the procedure.
Step 3: Assess the patient's forehead...
Assessing Body Temperature - Oral
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
Thermosensation
